"ALGAE-spirogyra", "look for chloroplast with green pigments, look for the nucleus" "PROTOZOA- amoeba", "look for the nucleus, amoeba crawl using pseudopods" "YEAST- S. cerevisiae", "These are unicellular fungi, look for the smaller pieces attached to the cells called buds (this is a type of asexual reproduction)" "MOLDS- Aspergillus", "these are multicellular fungi, look for the filamentous cells called hyphae, look for spores" "PRIMARY STAIN", "stains all cell the stain" "MORDANT", "enhances the primary stain and allows better penetration of stain into certain cell types" "DECOLORIZER", "removes color from some cells while leaving it in others" "COUNTERSTAIN", "adds a second round of stain to all cells" "GRAM STAIN", "determines type of cell wall" "ACID FAST STAIN", "specific for Mycobacterium" "ENDOSPORE STAIN", "specific for endospores in Bacillius and Clostridium" "SIMPLE STAIN", "capsule stain- Identifies bacteria with a capsule. Bacteria with capsules are identified as pathogens (they will cause diseases).<br /> Example: Streptococcus and Pseudomonas" "PREPARING SLIDES<br /> method1: Wet mount technique", "add sample in a drop of water to the slide<br /> add stain if needed<br /> cover with coverslip" "PREPARING SLIPED<br /> method2;", "add sample in a drop of water to a slide<br /> allow to air dry<br /> heat fix by passing several times quickly through bunsen burner flame<br /> -heat fixing attaches to cells to slide, kills the cell, open cell wall to allow stain to enter.<br /> add stains after heat fixing" "01.When doing a gram stain procedure, what color is a gram-negative cell after decolorized has been added ?", "The gram- negative cell is always pink" "02: In this diagram, why is the decolorized not forcing the color from the cell?", "The cell wall is too thick and has no priors to allow the larger CV-I complex out" "03: From this diagram, what is the thick, yellow layer surrounding the cell?", "The thick, yellow layer is the mycelia acid layer." "04:In the acid fast staining procedure, why does the methylene Blue not add to the cell’s layers and create a purple color?", "all options are correct" "05: In the Spore staining, what is the mordant in the procedure", "Heat" "06:In the spore staining, why does the spore not stain red like the cell?", "the spore has many layers of protection that keep chemicals out" "07: what genus of bacteria can be identified by a spore stain procedure?", "Bacillus or Clostridium" "08: If we identify spore on a spore stain slide, what type of environment were the cell in before the spore stain procedure to see these results", "The cell were in an unforvarable environment to create spore" "09:If we do a spore stain procedure and we see circular red cell, what do we know about these cells?", "circular cells will not make spore because they are not Bacillus or Clostridium" "10: what do we know about the cells that stain pink in a gram stain procedure", "we will know their cell wall is negative, we could also see shape and arrangement" "11.Looking at this diagram, what type of staining was done here?", "capsule stain" "12: when you look math this stained slide(blue and pink), what type of staining is this?", "Acid fast stain" "13: When you look at this stained slide(blue and pink), what disease might be associated with this slide?", "this may show us TB or Leprosy" "14: When you look at this stained slide(green and red), what type of staining is this?", "Spore Stain" "15: From this stained slide (green and red), what diseases might be associated with this slide?", "This may show Anthrax or tetanus"
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