"Primary stain crystal violet<br /> 01.What color is each cell after Crystal Violet is added?", "Gram positive bacteria retains crystal violet<br /> Gram negative bacteria is red or pink coloring." "Mordant:Iodine added to cell. Crystal Violet forms ionic bond with Iodine to form CV-I Complex<br /> 02. What color is each cell after added iodine?", "cells appear colorless." "Decolorized is added to cell<br /> 03.What color is each cell after decolorized is added", "the gram-positive cell remains purple in color<br /> gram negative cell loses the purple color and its only revealed when counterstain." "Counterstain, Safrain is added to cell<br /> 04.What color is each cell after safranin is added", "Gram-postiive: does not disrupt color purple coloration<br /> Gram-negative: cell stain red" "Step1: Primary stain Carbol fuchsin is added to each cell, this stain has an additional chemical, phenol, that helps the stain penetrate<br /> 5A. What and where is the mordant", "-A mordant is a chemical or physical agent that enhances the primary stain<br /> What is a mordant: Primary stain that binds with crystal violet<br /> -" "5b:What color is each cell?", "Gram Positive: dark crystal violet instead of the safranin cells appear purple<br /> Gram Negative: after ethanol it appears red" "5c:Identify the fast acid + and fast acid -", "Acid fast positive cells stain bright pink/magenta and are members of genus Mycobacterium<br /> Acid fast negative cells stain blue and are not members of the genus Mycobacterium" "Step2: Decolorizer is added to the cell<br /> 6A:What is decolorizer made of?", "Ethanol" "6B:What color is each cell?", "Pink" "Step3: Counterstain, methylene blue is added to the cell<br /> 7A:What color is each cell?", "Pink" "7B:Identify The acid fast cell", "Used to identify acid fast organisms such as members of the genus family mycobacterium" "7C:What else do you know about the Acid fast cell?", "Acid fast smear will have a mix of both pink and blue cells" "7D.What disease may be identified", "Mycobacterium"
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