"Primary stain crystal violet 01.What color is each cell after Crystal Violet is added?", "Gram positive bacteria retains crystal violet Gram negative bacteria is red or pink coloring." "Mordant:Iodine added to cell. Crystal Violet forms ionic bond with Iodine to form CV-I Complex 02. What color is each cell after added iodine?", "cells appear colorless." "Decolorized is added to cell 03.What color is each cell after decolorized is added", "the gram-positive cell remains purple in color gram negative cell loses the purple color and its only revealed when counterstain." "Counterstain, Safrain is added to cell 04.What color is each cell after safranin is added", "Gram-postiive: does not disrupt color purple coloration Gram-negative: cell stain red" "Step1: Primary stain Carbol fuchsin is added to each cell, this stain has an additional chemical, phenol, that helps the stain penetrate 5A. What and where is the mordant", "-A mordant is a chemical or physical agent that enhances the primary stain What is a mordant: Primary stain that binds with crystal violet -" "5b:What color is each cell?", "Gram Positive: dark crystal violet instead of the safranin cells appear purple Gram Negative: after ethanol it appears red" "5c:Identify the fast acid + and fast acid -", "Acid fast positive cells stain bright pink/magenta and are members of genus Mycobacterium Acid fast negative cells stain blue and are not members of the genus Mycobacterium" "Step2: Decolorizer is added to the cell 6A:What is decolorizer made of?", "Ethanol" "6B:What color is each cell?", "Pink" "Step3: Counterstain, methylene blue is added to the cell 7A:What color is each cell?", "Pink" "7B:Identify The acid fast cell", "Used to identify acid fast organisms such as members of the genus family mycobacterium" "7C:What else do you know about the Acid fast cell?", "Acid fast smear will have a mix of both pink and blue cells" "7D.What disease may be identified", "Mycobacterium" "Slide placed over hotplate for 5 mins. This heat is just strong enough to loosen the Peptidoglycan and protein layers surrounding the spore to allow the stain into the spore. 8A:What is the mordant", "Steam" "8B:What color is the cell?", "Red" "8C:What does the small circle In the cell represent?", "Green endospores" "8D:What color is that small circle?", "Green" "Spore Staining 9A:What is decolorized made of", "Water" "9B:What is the color of the cell after decolorized is added?", "Malachite Green" "9C:What color is of the small circle after decolorized is added?", "red" "10A:What is the color of the cell after Safranin is added?", "Cell stain red" "10B:What color is the small circle after safranin is added", "green" "10C:What do you know about who these cells might be?", "Bacillius or clostridium" "10D:What type of environment were these cells in? How do you know ?", "-These bacteria often live in soil, so it could be. a natural stress such as a drought that initiates their conversion into an endospore -Artificial stress, such as treatment with desinfectants, would have a similar effect." "10E: What disease may be seen with this", "Bacillius, clostridium" "11A:What are the clear circles?", "Capsule" "11B.What are the tiny dark circles?", "Bacterium" "11C:What do you know about the cells with the clear circles compared to the cells with no clear circle?", "-Capsule will resist stain and will make the cells that have capsule visible as white areas against the dark background -cells without a capsule will take in the dark stain and will match the background."
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